![]() ![]() The Redirect To feature allows you to outline a structure on one image and measure the intensity of the corresponding region in another image. Redirect To - The image selected from this popup menu will be used as the target for statistical calculations done by the Measure and Analyze Particles commands. Invert Y Coordinates - If checked, the XY origin is assumed to be the lower left corner of the image window instead of the upper left corner. Use Image>Adjust>Threshold to set the threshold limits.ĭisplay Label - If checked, the image name and slice number (for stacks) are recoded in the first column of the results table. Limit to Threshold - If checked, only thresholded pixels are included in measurement calculations. ![]() For non-thresholded images, the percentage of non-zero pixels. Kurtosis - The fourth order moment about the mean.Īrea Fraction - The percentage of pixels in the image or selection that have been highlighted in red using Image/Adjust/Threshold. The documentation for the Moment Calculator plugin explains how to interpret spatial moments. Skewness - The third order moment about the mean. Median - The median value of the pixels in the image or selection. The Dot Blot Analysis example demonstrates how to use this option to analyze a dot blot assay. This is equavalent to the product of Area and Mean Gray Value. Integrated Density - The sum of the values of the pixels in the image or selection. The Feret's Diameter macro will draw the Feret's Diameter of the current selection on the image. In those cases the value of 1.0 is returned (before v1.37 this was set as -1.0).įeret's Diameter - The longest distance between any two points along the selection boundary. For small particles, the circularity can be >1 (due to the definition of perimeter and area in a square grid). Values may not be valid for very small particles. As the value approaches 0.0, it indicates an increasingly elongated polygon. A value of 1.0 indicates a perfect circle. Note that ImageJ cannot calculate the major and minor axis lengths if Pixel Aspect Ratio in the Set Scale dialog is not 1.0.Ĭircularity - 4pi(area/perimeter^2). Major and Minor are the primary and seconday axis of the best fitting ellipse.Īngle is the angle between the primary axis and a line parallel to the x-axis of the image. Uses the headings Major, Minor and Angle. Uses the headings BX, BY, Width and Height, where BX and BY are the coordinates of the upper left corner of the rectangle.įit Ellipse - Fit an ellipse to the selection. Perimeter - The length of the outside boundary of the selection.īounding Rectangle - The smallest rectangle enclosing the selection. This is the average of the x and y coordinates of all of the pixels in the selection.Ĭenter of Mass - This is the brightness-weighted average of the x and y coordinates all pixels in the selection. Min & Max Gray Value - Minimum and maximum gray values within the selection.Ĭentroid - The center point of the selection. Corresponds to the highest peak in the histogram. Modal Gray Value - Most frequently occurring gray value within the selection. Standard Deviation - Standard deviation of the gray values used to generate the mean gray value. ![]() or the formula gray=(red+green+blue)/3 if not checked. For RGB images, the mean is calulated by converting each pixel to grayscale using the formula gray=0.299*red+0.587*green+0.114*blue if “ Weighted RGB Conversion” is checked in Edit>Options>Conversions. Reported in calibrated units (e.g., optical density) if Analyze>Calibrate was used to calibrate the image. This is the sum of the gray values of all the pixels in the selection divided by the number of pixels. Mean Gray Value - Average gray value within the selection. Area is in calibrated units, such as square millimeters, if Analyze>Set Scale was used to spatially calibrate the image. Use this dialog box to specify which measurements are recorded by Analyze/Measure and Analyze/Analyze Particles.Īrea - Area of selection in square pixels. ![]()
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